Methods Of A 200 Mg Caffeine Pill - 1093 Words

Methods A 200 mg caffeine pill was cut in half with a razor. Next, one of the halves was then crushed using a mortar and pestle; the final measurement was 100 mg of caffeine. The 100 mg of caffeine was then dumped into 100 ml of distilled water in a 250ml beaker. Using a stirring rod, the caffeine was then diffused into the water by stirring the mixture for ten minutes. Four 40 ml beakers were then collected and labeled with tape. The beakers were labeled as the control (0.0% caffeine), 0.1% of caffeine, 0.01% of caffeine, and 0.001% caffeine. 10 ml of distilled water was placed into the beaker labeled ‘control.’ The other three beakers were filled with 9ml of distilled water. 1 ml of the water-caffeine mixture was sucked into a pipette, and was then dropped into the beaker labeled 0.1% caffeine. 1 ml of the 0.1% caffeine was sucked into the pipette, and then placed in the beaker labeled 0.01% caffeine. 1 ml of of the 0.01% caffeine was then sucked into the pipette and placed i nto the beaker labeled 0.001% caffeine. Four test tubes were then collected and labeled with tape as control, 0.1%, 0.01%, and 0.001%. Using a sterile swab, the inside of a human mouth was swabbed. This was then mixed with 2 ml of distilled water. In each test tube, 0.5 ml of the bacteria solution was added with a pipette. Then, in the test tube labeled control, 0.5 ml of the distilled water was added. 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